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KMID : 0380620210530030334
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 3 p.334 ~ p.343
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16S rRNA gene-based sequencing of cucumber (Cucumis sativus L.) microbiota cultivated in South Korea
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Seo Dong-Woo
Kim Seung-Min
Lee Heoun-Reoul
Yum Su-Jin
Jeong Hee-Gon
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Abstract
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Various vegetables, including cucumbers, have a high probability of foodborne illness because they are usually eaten raw. In this study, we analyzed the 16S rRNA gene sequences of the cucumber (Cucumis sativus L.) microbiota.
The diversity indices of cucumber cultivated in May were higher than in cucumber cultivated in November. At the phylum level, Proteobacteria, Firmicutes, and Actinobacteria were predominant. The classes generally comprised Gammaproteobacteria, Bacilli, Alphaproteobacteria, and Actinobacteria. At the genus level, the proportions of Aureimonas, Escherichia, and Microbacterium in samples from May were relatively high, whereas Enterococcus, Pseudomonas, and Rhizobium accounted for a higher proportion in samples from November. Moreover, it is noteworthy that potential pathogenic genera such as Acinetobacter, Aerococcus, Aureimonas, Enterobacter, Enterococcus, Escherichia, Pantoea, Pseudomonas, and Staphylococcus were detected. Although further studies on the characteristics of potential pathogens are required, our results can be used to improve the food safety of vegetables.
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KEYWORD
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16S rRNA gene-based sequencing, Food safety, Cucumber, Microbiota
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